Emmanuel Macron and his wife, Brigitte, are planning to present photographic and scientific evidence in a US court to prove that Mrs. Macron is a woman.
Their lawyer says the French president and Mrs Macron will submit documentation in a defamation lawsuit they have filed against right-wing influencer Candace Owens after she promoted her belief that Brigitte Macron was born male.
Owens' attorneys have responded with a motion to dismiss the claim.
Speaking to the BBC's Fame Under Fire podcast, Macron's lawyer in the case, Tom Clare, said that Mrs Macron had found the allegations "extremely disturbing" and that they were a "distraction" for the French president.
"I don't want to suggest that this has somehow distracted him. But, like anyone who is dealing with a career and a family life, when your family is under attack, it wears you down. And he is not immune to that because he is the president of a country," he said.
Clare said there would be "expert evidence that will be of a scientific nature" and while he would not reveal, at this stage, the exact nature of it, he said the couple were prepared to fully demonstrate "both generally and specifically" that the allegations are false.
"It's extremely disturbing to think that you have to go and subject yourself, to present this kind of evidence," he said.
"It's a process that she will have to undergo in a very public way. But she is willing to do it. She is strongly determined to do what it takes to clarify the situation."
"If that resentment and that discomfort that she has from opening herself up in that way is what it takes to clarify the situation and stop this, she is 100% willing to shoulder that burden."